At 05H01 Wednesday morning Netcare 911 responded to reports of bus crash on the Moloto Road near Cullinan.
Reports from the scene indicate that the driver of a bus lost control resulting in a rollover.
Medics assessed the scene and found that two people had sadly sustained fatal injuries and were declared deceased on the scene.
Thirty-four other patients sustained injuries ranging form minor to moderate injuries.
All the patients were treated on scene and once stabilised were transported by various ambulance services to hospital for further treatment.
Circumstances leading up to the collision will be investigated by the relevant authorities.